4 Hybridization

Production of genetically male tilapia through interspecific hybridization between Oreochromis niloticus and O. aureus

This study was conducted to produce a high percentage of genetically male tilapia through interspecific hybridization between Nile tilapia, Oreochromis niloticus and Blue tilapia, O. aureus and evaluate sex ratio, productive performance and heterosis of the progeny produced. The results revealed that sex ratios of the progenies of (♀ O. aureus x ♂ O. niloticus) and (♀ O. niloticus x ♂ O. aureus...

کاربرد PCR-Hybridization در تشخیص عفونتهای ناشی از مایکوپلاسما پنومونیه

Twenty to thirty percent of causative agents of the respiratory tract infections are still unknown, that can be related to the lack of suitable laboratory procedures. Some of these organisms may cause progressive infection such as encephalitis and cardiac complications. To recognize the M. pneumoniae , PCR-hybridization technique was used and a specific internal probe was produced for this...

Intraspecific Hybridization of Populus euphratica Oliv. Using in vitro Technique

Hybridization of Cuckoo Search and Firefly Algorithms to Calculate the Interaction Parameters in Phase Equilibrium Modeling Problems

Liquid-liquid equilibrium (LLE) problems such as phase stability analysis, phase equilibrium calculations, chemical equilibrium calculations, binary interaction parameter identification of thermodynamic models and other problems of fluid characterization have been the core subject of many recent studies. This study introduces Cuckoo Search (CS), Firefly Algorithms (FA) and its variants as p...

دورگه‌سازی در محل؛ اصول و کاربردها : مقاله مروری

In situ hybridization (ISH) is a method that uses labeled complementary single strand DNA or RNA to localize specific DNA or RNA sequences in an intact cell or in a fixed tissue section. The main steps of ISH consist of: probe selection, tissue or sample preparation, pre-hybridization treatment, hybridization and washing, detection and control procedure. Probe selection is one of the important ...

Gene Probe Designing for Evaluation of the Diversity of Bradyrhizobium japonicum Isolates

Many researchers consider the use of different probes for hybridization assays as suitable for studying the genetic diversity of nitrogen fixing bacteria. In this study for asessing genetic diversity among Bradyrhizobium japonicum isolates, two different probes (sucA and topA) chosen from the chromosomal genome of Bradyrhizobium strain USDA 110 were designed, evaluated by DNAMAN software and im...